magnetic separator using bacteria

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magnetic separator using bacteria

magnetic separator using bacteria

[Separation of magnetic bacteria by using a magnetic

2010-2-1  A magnetic separator was used to separate magnetic bacteria based on their magnetotactic characteristics. Acidithiobacillus ferrooxidans, a bacterium that could synthesize intra-cellular nanometer magnetic particles, was investigated as an example. Strong magnetic and weak magnetic cells were separated and collected.

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Development of a magnetic separation method to

2016-9-1  We used a mutated lysozyme coupled to magnetic beads (LysE35A-bead) for the specific capture and separation of bacteria. Magnetic separation is efficient in removing inhibitory substances from blood and isolates the target cells directly from samples without the need of standard concentration steps (Olsvik et al., 1994). In addition, this method is non-destructive for the target cells, which allow subsequent detection of the bacteria.

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Magnetic Cell Separation Using Antibody Binding with

2004-10-7  Bacterial magnetic particles (BacMPs) are efficient platforms of proteins for surface display systems. In this study, mononuclear cells from peripheral blood were separated using BacMPs expressing protein A on the BacMP membrane surface (protein A−BacMPs), which were complexed with the Fc fragment of anti-mouse IgG antibody.

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(PDF) A Fluidic Device for Immunomagnetic

2018-11-27  Under the optimal conditions, the target bacteria could be separated with a separation efficiency of 80% in 45 min. This fluidic device could be further improved using thinner sawtooth-shaped iron...

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Selective, Agglomerate-Free Separation of Bacteria

Selective, Agglomerate-Free Separation of Bacteria Using Biofunctionalized, Magnetic Janus Nanoparticles

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Isolation and separation of Listeria monocytogenes

2019-3-1  A bacteriophage-assisted magnetic separation method was developed for the isolation of Listeria monocytogenes from complex food matrices. The aim of this study is to understand the effect of phage immobilization methods and the magnetic particle sizes on the phage coupling and infectivity retention of the magnetic particles.

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A colorimetric immunosensor for determination of

2020-3-3  The new rotating magnetic separator was demonstrated to be effective for continuous-flow separation of target bacteria from large volume using a small amount of magnetic nanoparticles. However, the separation time is a little long and might be shortened using larger-sized magnetic nanoparticles with faster flow rate and stronger magnetic field.

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Rapid fluorescence detection of pathogenic bacteria

2017-6-29  Centrifugation, filtration, and magnetic separation were commonly used for rapid bacterial separation and enrichment from samples [ 8 ]. However, centrifugation and filtration may cause the loss of bacteria when only very few bacteria existed.

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Quantitative separation of bacteria in saline solution

Bacteria were separated from solution inside a capillary flow cell exposed to a highly non-homogeneous magnetic field (maximum field intensity was 0.4 T) and quantified by a light scattering method.

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Simple Colorimetric Assay for Vibrio parahaemolyticus

2020-9-1  Magnetic separation (MS) technology is one of the most popular techniques for isolation and enrichment of bac- teria.17−23Compared with traditional separation techniques, magnetic separation has many advantages enabling bacteria to

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A novel TiO2-assisted magnetic nanoparticle

A novel TiO 2-assisted magnetic nanoparticle separator for treatment and inactivation of bacterial contaminants in aquatic systems Werner E. G. Müller ,* a Thorben Link,a Qiang Li,b Heinz C. Schröder,a Renato Batel,c Maria Blažina,c Vladislav A.

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Multiple sample flow through immunomagnetic

2005-6-1  Both series of experiments used 0.02 ml of magnetic beads. The results show that a much higher number of bacteria are recovered using the MSFTIMS separator, but the system has insufficient sensitivity for low bacterial dilutions (e.g. <20 cfu ml −1) when used with small volume of beads (0.02 ml beads/250 ml sample). Therefore, further

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Advection Flows‐Enhanced Magnetic Separation for

2018-7-25  Advection Flows‐Enhanced Magnetic Separation for High‐Throughput Bacteria Separation from Undiluted Whole Blood Su Hyun Jung Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST gil 50, Ulsan, 44919 Republic of Korea

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Advection Flows‐Enhanced Magnetic Separation for

2018-7-25  Advection Flows-Enhanced Magnetic Separation for High-Throughput Bacteria Separation from Undiluted Whole Blood Su Hyun Jung,Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST gil 50, Ulsan, 44919 Republic of Korea

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Cobalt separation by Alphaproteobacterium MTB

2016-8-8  Bioremediation of toxic metals by magnetotactic bacteria and magnetic separation of metal-loaded magnetotactic bacteria are of great interest. This bioprocess technique is rapid, efficient, economical, and environmentally friendly. In this study, cobalt removal potential of a novel isolated magnetotactic bacterium (Alphaproteobacterium MTB-KTN90) as a new biosorbent was investigated.

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Simple Colorimetric Assay for Vibrio parahaemolyticus

2020-9-1  Magnetic separation (MS) technology is one of the most popular techniques for isolation and enrichment of bac-teria.17−23 Compared with traditional separation techniques, magnetic separation has many advantages enabling bacteria to be quickly localized or retrieved with a common magnet. Using the magnetic nanoparticles (MNPs) modified with

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Ultrarapid Detection of Pathogenic Bacteria Using a

2019-5-24  Nearly perfect capture of the AbMNCs and AbMNCs-Salmonella complexes was achieved under a high flow rate by stacking permanent magnets with spacers inside the cylindrical separator to maximize the magnetic force. The concentration of the bacteria in solution was determined using ATP luminescence measurements.

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Rapid fluorescence detection of pathogenic bacteria

2017-6-29  A rapid and sensitive analytical method was developed to detect pathogenic bacteria which combined magnetic enrichment, fluorescence labeling with polyethylene glycol (PEG) magnetophoretic chromatography. As pathogenic bacteria usually exist in complex matrixes at low concentration, an efficient enrichment is essential for diagnosis. In order to capture series types of pathogenic bacteria

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The basic guide to magnetic bead cell separation

2017-10-8  Magnetic cell separation entails the action of magne tic force on cellular particles blood, bone marrow, cultivation media, food, soil, stool, tissue-homoge-nates, water, and so forth set in solution. Magnetic force applied to the solution separates treated cells (those with magnetic beads attached) from untrea-

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A novel TiO2-assisted magnetic nanoparticle

A novel TiO 2-assisted magnetic nanoparticle separator for treatment and inactivation of bacterial contaminants in aquatic systems Werner E. G. Müller ,* a Thorben Link,a Qiang Li,b Heinz C. Schröder,a Renato Batel,c Maria Blažina,c Vladislav A.

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Advection Flows‐Enhanced Magnetic Separation for

2018-7-25  Advection Flows‐Enhanced Magnetic Separation for High‐Throughput Bacteria Separation from Undiluted Whole Blood Su Hyun Jung Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST gil 50, Ulsan, 44919 Republic of Korea

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Advection Flows‐Enhanced Magnetic Separation for

2018-7-25  Advection Flows-Enhanced Magnetic Separation for High-Throughput Bacteria Separation from Undiluted Whole Blood Su Hyun Jung,Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST gil 50, Ulsan, 44919 Republic of Korea

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Cobalt separation by Alphaproteobacterium MTB

2016-8-8  Bioremediation of toxic metals by magnetotactic bacteria and magnetic separation of metal-loaded magnetotactic bacteria are of great interest. This bioprocess technique is rapid, efficient, economical, and environmentally friendly. In this study, cobalt removal potential of a novel isolated magnetotactic bacterium (Alphaproteobacterium MTB-KTN90) as a new biosorbent was investigated.

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Magnetic Beads gDNA Kit (Bacteria) Geneaid

2020-12-3  magnetic separator for 30 seconds or until ME Magnetic Beads have pelleted. Remove and discard the supernatant. Add 600 μl of MW2 Buffer (make sure ethanol was added) and gently shake the tube for 1 minute. Place the tube in a magnetic separator for 30 seconds or until ME Magnetic Beads have pelleted. Remove and discard the supernatant. 5.

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Simple Colorimetric Assay for Vibrio parahaemolyticus

2020-9-1  Magnetic separation (MS) technology is one of the most popular techniques for isolation and enrichment of bac-teria.17−23 Compared with traditional separation techniques, magnetic separation has many advantages enabling bacteria to be quickly localized or retrieved with a common magnet. Using the magnetic nanoparticles (MNPs) modified with

get price

Ultrarapid Detection of Pathogenic Bacteria Using a

2019-5-24  Nearly perfect capture of the AbMNCs and AbMNCs-Salmonella complexes was achieved under a high flow rate by stacking permanent magnets with spacers inside the cylindrical separator to maximize the magnetic force. The concentration of the bacteria in solution was determined using ATP luminescence measurements.

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Electroosmotic flow driven microfluidic device for

2019-10-2  An on-chip magnetic bead separator using spiral electromagnets with semi-encapsulated permalloy. Biosensors and Bioelectronics 16,409–416 (2001). CAS Article Google Scholar

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A magnetic relaxation DNA biosensor for rapid

A magnetic relaxation DNA biosensor was constructed for rapid detection of Listeria monocytogenes.. The strategy of dual-probe hybridization and ALP-mediated MnO 4-M/Mn 2+ conversion was proposed.. The method can specifically distinguish Listeria monocytogenes with a LOD of 10 2 CFU mL −1.. This biosensor provided a potential application for other pathogenic bacteria.

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